Antioxidant activity, total phenolic content and cytotoxicity of polar extracts from Colombian antidiabetic plants

Revista Cubana de Plantas Medicinales

Balcázar Morales, Norman¹  Rivas Mena, Kevin Eduardo²  MSc. Pino Benítez, Cruz Nayive²  Muñoz, Diana Lorena¹ 

[1] Universidad de Antioquia, Medellín, Colombia;Universidad Tecnológica del Chocó, Quibdó, Colombia

关键词: traditional medicine;    antidiabetic plants;    antioxidant;    phenolic compounds;    cytotoxic;    Hep-G2;    C2C12;   

DOI  :  

学科分类：药理学

来源: Editorial Ciencias Medicas

【 摘 要 】

Introduction:the traditional use of plants as antidiabetic herbal medicine is common practice in the Colombian Pacific, A. altilis, B. picta, S. malaccensis, P. microphylla y P. quadrangularis are five species that are widely recognized as natural antidiabetic regionally, without the existence experimental evidence to validate that assessment.Objective:evaluate the total content of phenolic compounds, potential antioxidant and cytotoxic activity of polar extracts prepared from five plant species used as natural antidiabetic Colombian traditional medicine.Methods:extracts of leaves (leaves and stems for P. microphylla) were obtained by maceration in 96 % ethanol or distilled H2O as appropriate. The antioxidant activity was evaluated by spectrophotometric assays DPPH 2, 2-diphenyl-1-picrilhidracil and ABTS 2, 2 '-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid), while the total content of phenolic molecules is determined by the Folin-Ciocalteu method. Furthermore, the cytotoxic activity was tested in the Hep-G2 cell line (human hepatocarcinoma) and C2C12 (mouse Miocarcinoma).Results: the ethanol extracts of A. altilis, S. malaccensis and B. picta, showed the best antioxidant behavior in both methods, with properties comparable to vitamins E and C. Likewise, as to the toxic properties of the extracts A. altilis reported the highest percentage of mortality in C 2C12 cells with an LC50 value of 24,9 ± 0,67 mg/ mL, comparable to the cytotoxicity exerted by amphotericin B, while in HEP-G2 cells the major toxic effect was governed by P. quadrangularis with LC50 of 36,4 ± 5,79 mg/mL.Conclusions:the potential antioxidant evidenced by the ethanol and aqueous extracts of A. altilis, S. malaccensis, B. picta, P. quadrangularis and P. microphylla in 62.39% correlated with the total content of phenolic molecules. All extracts showed fifty cytotoxic activitiesat concentrations greater than their antioxidant activityrange.

【 授权许可】

CC BY-NC