Optimization of culture conditions of Streptomyces rochei (MTCC 10109) for the production of antimicrobial metabolites

Egyptian Journal of Biology

SV Rajagopal¹  DPN Ramakrishna¹  NG Reddy¹ 

关键词: Botanicals;    calcitonin;    calcium;    teleost;   

DOI  :  

学科分类：生物科学（综合）

来源: Egyptian-English Society for Biological Sciences

【 摘 要 】

Fermentation and culture conditions were studied in shaken-flask culture to induce the production of greater amounts of antimicrobial metabolites by Streptomyces rochei (10109). Antimicrobial metabolite production started after 48 h incubation and reached its optimum level at 20% inoculum size at 120 h, at which point the metabolites showed maximum antifungal and antibacterial activity against selected human pathogenic microorganisms ( Candida albicans, Staphylococcus aureusandEscherichia coli ). Optimal production occurred at pH 7.5 and temperature 32°C, with 2% glycerol and 1% peptone as the carbon and nitrogen sources respectively. The effects of adding sea water (optimum 30%) and NaCl (optimum 1%) were also evaluated. Keywords:human pathogens

【 授权许可】

Unknown