Experimental induction of gill disease in Atlantic salmon Salmo salar smolts withTenacibaculum maritimum

Diseases of Aquatic Organisms

Mark Powell¹  Jeremy Carson¹  Rebecca van Gelderen¹ 

关键词: Atlantic salmon;    Tenacibaculum maritimum;    Pathophysiology;    Gill disease;    Osmoregulation;    Respiration;   

DOI  :  10.3354/dao061179

学科分类：生物科学（综合）

来源: Inter-Research

【 摘 要 】

ABSTRACT: An experimentally induced bacterial infection of marine Atlantic salmon Salmo salar smolt gills was developed using strains of Tenacibaculum maritimum originally isolated from disease outbreaks in Tasmania. The gills of salmon wereinoculated with a high concentration of bacteria (4 × 10¹¹ cells per fish) of either strain 00/3280 or 89/4747 T. maritimum. Gentle abrasion of the gills was used to enhance the progression of gill disease. One strain (00/3280) washighly pathogenic, causing morbidity and mortality within 24 h post-inoculation, and produced acute focal branchial necrosis associated with significant increases in plasma osmolality and lactate concentration compared with controls (non-inoculated) orstrain 89/4747-inoculated fish. There were no differences in the whole body net ammonium flux between control (non-inoculated) and strain 00/3820-inoculated fish. Gill abrasion resulted in acute telangiectasis and focal lamellar hyperplasia in all fishregardless of bacterial inoculation. This work provides the basis of a challenge model suitable for investigating the pathophysiological processes associated with acute branchial necrosis in marine fish, suggesting that osmoregulatory and possiblyrespiratory dysfunction are the primary consequences of infection.

【 授权许可】

Unknown