Annexin 5 Inhibits Thyrotropin Releasing Hormone (TRH) Stimulated Prolactin Release in the Primary Culture of Rat Anterior Pituitary Cells

Endocrine Journal

Akio SENDA¹  Inoru HASHIMOTO¹  Mitsumori KAWAMINAMI¹  Seishi ETOH¹  Hiroko MIYAOKA¹  Shiro KURUSU¹ 

[1] Laboratory of Veterinary Physiology, School of Veterinary Medicine and Animal Sciences, Kitasato University

关键词: Annexin 5;    Prolactin;    Pituitary;    TRH;    Indomethacin;   

DOI  :  10.1507/endocrj.51.349

学科分类：内分泌与代谢学

来源: Japan Endocrine Society

【 摘 要 】

References(30)Cited-By(1)Annexin 5, a novel calcium-phospholipid binding protein, is thought to be involved in hormone secretion by the anterior pituitary gland. Gonadotropin releasing hormone stimulates annexin 5 synthesis, which, in turn, enhances gonadotoropin secretion. On the other hand, annexin 5 was shown to inhibit prolactin release in vitro. To understand the nature of the opposing effects of annexin 5 on these two major pituitary hormones, the present study examines the inhibitory effect of annexin 5 on prolactin release in relation to thyrotropin stimulating hormone (TRH) using primary cultures of anterior pituitary cells of adult female rats. While recombinant rat annexin 5 was found to have little effect on basal prolactin release, it significantly inhibited TRH-stimulated prolactin release. Addition of specific anti-annexin 5 serum to the culture increased basal prolactin release in a concentration dependent manner, and no further increase in prolactin release was observed following application of TRH in the presence of anti-annexin 5. The enhanced basal prolactin release induced by anti-annexin 5 was reversed by the simultaneous administration of indomethacin, an inhibitor of cyclooxygenase. These results demonstrate that endogenous pituitary annexin 5 exerts an inhibitory effect on prolactin release and suggest that this is attained by suppression of eicosanoid synthesis in vitro.

【 授权许可】

Unknown