In vitro photohemolytic assessment of Cissus sicyoides L. y Achyranthes aspera

Revista Cubana de Plantas Medicinales

Boffill Cárdenas, María de los A.¹  Castillo Alfonso, Orestes¹  Martínez Montalbán, Celia M.¹  González Madariaga, Yisel¹  Bermúdez Toledo, Deodelsys¹  Martínez Bernal, Yusnel¹ 

[1] Unidad de Toxicología Experimental, Cuba

关键词: : photohemolysis;    erythrocytes;    in vitro;    Cissus sicyoides;    Achyranthes aspera;   

DOI  :  

学科分类：药理学

来源: Editorial Ciencias Medicas

【 摘 要 】

INTRODUCTION: erythrocytes are useful cells to identify potentially phototoxic agents provided by systemic administration as well as to study the phototoxicity mechanisms involving oxidative stress processes. OBJECTIVE: to evaluate the photohemolytic effect of soft extracts from aerial parts of Cissus sicyoides L. (Vitaceae) and Achyranthes aspera L. (Amaranthaceae). METHODS: an in vitro protocol using human erythrocytes as biological model; they were ultraviolet light-radiated for 90 minutes to evaluate damage in erythrocyte membranes on the basis of detected hemoglobin released into the medium. RESULTS: mild hemolysis was observed, being the photohemolytic effect lower than that of positive controls. CONCLUSIONS: the extracts from these plants were rated as non-irritating, which suggests that observed hemolysis may be caused by unstable erythrocyte membranes resulting from the existence of different metabolites in the studied extracts.

【 授权许可】

CC BY-NC