Virulence characterization of Campylobacter jejuni isolated from resident wild birds in Tokachi area, Japan

Journal of Veterinary Medical Science

Yoshiki OKOUCHI²  Anselme SHYAKA²  Aya YOSHIMURA¹  Keiko KAWAMOTO²  Akiko KUSUMOTO²  Warangkhana CHAISOWWONG²  Shinya FUKUMOTO¹ 

[1] Research Unit for Vector Biology, National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, 2�?11 Inada, Obihiro, Hokkaido 080�?8555, Japan;Section of Food Microbiology and Immunology, Diagnostic Center for Animal Health and Food Safety, Obihiro University of Agriculture and Veterinary Medicine, 2�?11 Inada, Obihiro, Hokkaido 080�?8555, Japan

关键词: Campylobacter;    Japan;    virulence;    wild bird;   

DOI  :  10.1292/jvms.15-0090

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(41)Cited-By(1)The prevalence of Campylobacter jejuni in wild birds is a potential hazard for human and animal health. The aim of this study was to establish the prevalence of C. jejuni in wild birds in Tokachi area, Hokkaido, Japan and investigate their virulence in vitro. In total, 173 cloacal swabs from individual wild birds were collected for the detection of Campylobacter spp. Thirty four samples (19.7%) were positive for Campylobacter of which 94.1% (32/34 samples) were C. jejuni. Additionally, one C. coli and one C. fetus were isolated. Seven C. jejuni isolates (one from crows and the other from pigeons) had important virulence genes including all three CDT genes (cdtA, cdtB and cdtC) and flaA, flaB, ciaB and cadF, and the other isolates were lacking cdtA gene. Further studies on in vitro virulence-associated phenotypes, such as motility assay on soft agar and invasion assay in Caco-2 cells, were performed. The wild bird C. jejuni isolates adhered and invaded human cells. Although the numbers of viable intracellular bacteria of wild bird isolates were lower than a type strain NCTC11168, they persisted at 48-hr and underwent replication in host cells.

【 授权许可】

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