Expression of LacZ Gene in Canine Muscle by Intramuscular Inoculation of a Plasmid DNA

Journal of Veterinary Medical Science

Kenichi MASUDA¹  Koichi OHNO¹  Hajime TSUJIMOTO¹  Keitaro OHMORI¹  Keigo KURATA²  Sadatoshi MAEDA¹  Masahiro SAKAGUCHI² 

[1] Department of Veterinary Internal Medicine, Graduate School of Agricultural and Life Sciences, The University of Tokyo;Department of Immunology, National Institute of Infectious Diseases

关键词: canine;    DNA immunization;    expression of β-galactosidase;   

DOI  :  10.1292/jvms.66.337

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(9)Cited-By(1)DNA immunization induces systemic humoral and cellular immune responses to the antigen encoded by cDNA in a plasmid DNA. In the present study, a plasmid DNA encoding cDNA of β-galactosidase (β-gal), pCAGGS-lacZ, was inoculated intramuscularly to a healthy dog in order to evaluate location and duration of the gene expression. On day 7, the plasmid DNA was found by PCR in the muscle where the plasmid was injected. Furthermore, β-gal expression was detected in the same muscle sample by β-gal staining. However, the plasmid DNA was not detected in any samples collected on days 14, 21 and 28. The present results suggest that duration of the gene expression of β-gal by the plasmid DNA is limited in the muscle in dogs and an efficacy for a gene expression should be evaluated depending on the gene inserted in the plasmid DNA for immunotherapy.

【 授权许可】

Unknown