期刊论文详细信息
Memórias do Instituto Oswaldo Cruz
Additives and Protein-DNA Combinations Modulate the Humoral Immune Response Elicited by a Hepatitis C Virus Core-encoding Plasmid in Mice
Liz Alvarez-lajonchere2  Santiago Dueñas-carrera2  Ariel Viña2  Thelvia Ramos1  Dagmara Pichardo2  Juan Morales2 
[1] ,Centro de Ingeniería Genética y Biotecnología Vaccine Division HCV Department
关键词: hepatitis C virus;    DNA immunization;    core;   
DOI  :  10.1590/S0074-02762002000100016
来源: SciELO
PDF
【 摘 要 】

Humoral and cellular immune responses are currently induced against hepatitis C virus (HCV) core following vaccination with core-encoding plasmids. However, the anti-core antibody response is frequently weak or transient. In this paper, we evaluated the effect of different additives and DNA-protein combinations on the anti-core antibody response. BALB/c mice were intramuscularly injected with an expression plasmid (pIDKCo), encoding a C-terminal truncated variant of the HCV core protein, alone or combined with CaCl2, PEG 6000, Freund's adjuvant, sonicated calf thymus DNA and a recombinant core protein (Co.120). Mixture of pIDKCo with PEG 6000 and Freund's adjuvant accelerated the development of the anti-core Ab response. Combination with PEG 6000 also induced a bias to IgG2a subclass predominance among anti-core antibodies. The kinetics, IgG2a/IgG1 ratio and epitope specificity of the anti-core antibody response elicited by Co.120 alone or combined with pIDKCo was different regarding that induced by the pIDKCo alone. Our data indicate that the antibody response induced following DNA immunization can be modified by formulation strategies.

【 授权许可】

CC BY   
 All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License

【 预 览 】
附件列表
Files Size Format View
RO202005130046126ZK.pdf 43KB PDF download
  文献评价指标  
  下载次数:1次 浏览次数:1次