Molecular markers in erva-mate (Ilex paraguariensis araguariensis St.Hil.) characterization studies: the taste

Ciência Rural

Ruiz, César Pérez²  Vidor, Mario Angelo¹  Moreno, Santiago Vázquez²  Floss, Paulo Alfonso³ 

[1] Empresa de Pesquisa Agropecuária e Extensão Rural de Santa Catarina, Lages;Universidad Politécnica de Madrid, Madrid, España;Centro de Pesquisas para Pequenas Propriedades, Chapecó

关键词: /font>Ilex paraguariensis;    molecular markers;    genetic variability;    PCR;    RAPD;    DNA;    biothecnology;   

DOI  :  10.1590/S0103-84782002000300008

学科分类：农业科学（综合）

来源: Universidade Federal de Santa Maria * Centro de Ciencias Rurais

【 摘 要 】

The Santa Catarina State Agricultural Research and Extensios Organization – Epagri SA, owns one of the largest Brazilian colletions or erva-mate (Ilex paraguariensis St.Hil.). Preliminary agronomic trials identified two promissing erva-mate materials named Barão de Cotegipe and Água Doce. Different tastes within each material were also identified, i.e. tastes with different grades of bitterness, apparently associated with the shape of leaves (CL – short and wide; LE – long and narrow). This study aimed to associate this plant patterns using molecular markers with plant origins and desirable fenotipic characteristics such as plant height, crown diameter, leaf density, plant survival, regrowth behaviour, within each taste group. For this molecular markers which amplify DNA of RAPD type (Random Amplified Polimorphic DNA) was used. The results did not allow, with the number of starters used as molecular markers, the genetic definition of the caracter taste, even though the results suggested so.

【 授权许可】

CC BY-NC