Role of Actin Microfilaments in Canine Distemper Virus Replication in Vero Cells

Journal of Veterinary Medical Science

Chieko KAI²  Hiroshi OZAKI¹  Hideaki KARAKI¹  Masahiro KAJITA¹  Masatoshi HORI¹  Kenjiro OHASHI²  Koichi SATO¹  Hideo KATAYAMA¹ 

[1] Department of Veterinary Pharmacology, Graduate School of Agriculture and Life Sciences, The University of Tokyo;The Laboratory Animal Research Center, Institute of Medical Science, The University of Tokyo

关键词: actin filament;    canine distemper virus;    protein expression;    Vero cell;    virus infection;   

DOI  :  10.1292/jvms.66.409

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(22)Cited-By(4)Several studies have indicated that viruses require a specific cytoskeletal structure for replication in host cells. In this study, we examined the role of actin fiber in the replication of canine distemper virus (CDV), belonging to the Morbillivirus genus of the family Paramyxoviridae. For this purpose, we used two actin depolymerizing agents, cytochalasin-D (C-D) and mycalolide-B (ML-B). In Vero cells, C-D disrupted actin fibers distributed in the cytosol, but peripheral actin fibers remained intact. On the other hand, ML-B completely disrupted the actin fibers distributed in both areas. Treatment of Vero cells with C-D or ML-B inhibited the replication of CDV. Double staining of CDV-infected Vero cells with antibody to N-protein and rhodamine-phalloidin revealed the presence of N-protein in mid-cytoplasm. However, the N-protein was specifically localized at the submembrane region in the presence of C-D, whereas it was clustered in the presence of ML-B. Viral mRNA levels of N- and H-proteins were rather increased by treatment with C-D or ML-B. The treatment with ML-B strongly inhibited N-protein expression, whereas C-D only slightly inhibited N-protein expression. These results suggest that actin microfilaments distributed in the cytoplasm and on the membrane region in host cells may have a different role in the process of CDV replication.

【 授权许可】

Unknown