The Growth Profiles of Three Types of Canine Distemper Virus on Vero Cells Expressing Canine Signaling Lymphocyte Activation Molecule

Journal of Veterinary Medical Science

Atsushi KATO³  Nguyen Thi LAN¹  Kazuyuki UCHIDA¹  Ryoji YAMAGUCHI¹  Kazushige KAI²  Susumu TATEYAMA¹ 

[1] Department of Veterinary Pathology, Faculty of Agriculture, University of Miyazaki;Department of Veterinary Microbiology, Faculty of Agriculture, Yamaguchi University;Department of Virology 3, National Institute of Infectious Diseases

关键词: CDV;    CPE;    growth properties;    Vero cell;    Vero-DST cell;   

DOI  :  10.1292/jvms.67.491

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(22)Cited-By(4)To know growth profiles of canine distemper virus (CDV) on Vero cells stably expressing canine signaling lymphocyte activation molecule (Vero-DogSLAMtag; Vero-DST cells), the propagation of three strains of CDV was tested in Vero-DST cells in comparison with parental Vero cells. Strain MD77 could grow well in both cell lines, but demonstrated no syncytium formation or indistinguishable rounding cytopathic effects (CPE) in Vero cells. Strains Onderstepoort and KDK-1 also grew well in Vero-DST cells with apparent syncytium CPE, while they grew less or no efficiently, respectively, in Vero cells. All three CDV strains demonstrated the peak titers, in Vero-DST cells before reaching to an extensive CPE and drastic decrease of titers at/after full CPE. Immunohistochemistry revealed that viral antigens of all CDV strains were found exclusively in the syncytia in Vero-DST cells, while in Vero cells, viral antigen was identified in their single cells for strain MD77 but none for other strains. Thus, every strain of CDV could grow well in Vero-DST cells and behaved differently against Vero cells. These results would be of practical value for workers of CDV because 1) In Vero-DST cells, by observation of distinct syncytium CPE, the highest titer or the best growth of virus could be identified; 2) In Vero cells, various CDV strains could be readily classified after propagation in Vero-DST cells.

【 授权许可】

Unknown