Proteome Analysis of Cerebrospinal Fluid in Healthy Beagles and Canine Encephalitis

Journal of Veterinary Medical Science

Kozo NAKAMURA¹  Sachiko NOMURA²  Taku MIYASHO²  Tetsuya NAKADE¹  Hiroshi YOKOTA² 

[1] Department of Small Animal Clinical Sciences, School of Veterinary Medicine, Rakuno Gakuen University, 582 Bunkyodai-Midorimachi, Ebetsu, Hokkaido 069-8501, Japan;Department of Veterinary Biochemistry, School of Veterinary Medicine, Rakuno Gakuen University, 582 Bunkyodai-Midorimachi, Ebetsu, Hokkaido 069-8501, Japan

关键词: 2DE;    canine;    CSF;    encephalitis;    proteome;   

DOI  :  10.1292/jvms.11-0474

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(19)Cited-By(7)We performed proteomics analysis of the cerebrospinal fluid (CSF) of healthy dogs and dogs with meningoencephalitis of unknown etiology (MUE). By comparing two-dimensional electrophoreses (2DE), an upregulated spot was found in MUE dogs. This protein was identified as a neuron-specific enolase (NSE) by analysis with MALDI-TOF mass spectrometry. In comparing dot blots using an antibody against NSE, the NSE levels in the CSF of MUE dogs was significantly higher than that of the controls. NSE is a diagnostic marker of neuroendocrine tumors, brain injury and spinal cord trauma in humans. It seems that the NSE concentration in the CSF is increased by cellular destruction in canine encephalitis. Though elevation of NSE may not be specific in canine encephalitis because the NSE level was increased in other CNS diseases, further study including measurement with serum is necessary.

【 授权许可】

Unknown