Expression Patterns of the slit Subfamily mRNA in Canine Malignant Mammary Tumors

Journal of Veterinary Medical Science

Toshihiko TANNO³  Kihei KUBO²  Hideo AKIYOSHI⁴  Shingo TSUYAMA³  Jyoji YAMATE¹  Takaaki SUGIURA²  Mitsuru KUWAMURA¹  Shigeo TAKENAKA³  Yuchie TANAKA³  Fumihito OHASHI⁴ 

[1] Laboratory of Veterinary Pathology, Department of Veterinary Science, Graduate School of Life and Environmental Sciences, Osaka Prefecture University;Laboratory of Veterinary Radiology, Department of Veterinary Science, Graduate School of Life and Environmental Sciences, Osaka Prefecture University;Laboratory of Cellular and Molecular Biology, Department of Veterinary Science, Graduate School of Life and Environmental Sciences, Osaka Prefecture University;Laboratory of Veterinary Surgery, Department of Veterinary Science, Graduate School of Life and Environmental Sciences, Osaka Prefecture University

关键词: canine mammary gland tumor;    malignancy;    robo;    slit;    tumor marker;   

DOI  :  10.1292/jvms.68.1173

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(20)Cited-By(3)Slit, a secreted protein, functions as a chemorepellent factor in axon guidance and neuronal migration and as an inhibitor in leukocyte chemotaxis. In humans, slit2 protein attracts endothelial cells and promotes tube formation in the tumor angiogenic mechanism. In this study, we cloned a part of the canine slit subfamily and examined the expression of slit subfamily mRNAs in 3 normal canine mammary glands and 11 mammary tumor samples by RT-PCR. The cloned part of the slit gene sequences showed high similarity to those of the human, mouse, and rat. The mRNAs were expressed at low levels in the normal mammary gland. The expression levels of slit1 mRNA were low in both the normal and tumor tissues. In contrast, the expression of slit2 mRNA increased in most of the malignant mammary tumors, and an increase in slit3 mRNA expression was observed in 2 of the malignant mixed tumors. These results suggest that the expression of slit2 plays an important role in tumor angiogenesis in canine mammary gland tumors and that slit2 can be a putative marker for malignancy diagnosis of these tumors.

【 授权许可】

Unknown