Sequence Variation of Dengue Type 2 Virus Isolated from Clinical Cases in Thailand

Japanese Journal of Infectious Diseases

Atchareeya A-nuegoonpipat³  Kazuyoshi Ikuta¹  Panjaporn Chaichana¹  Surapee Anantapreecha³  Chidchanok Khamlert¹  Akifumi Yamashita²  Takeshi Kurosu¹  Supranee Phanthanawiboon¹ 

[1] Research Collaboration Center on Emerging and Re-emerging Infections (RCC-ERI), Research Institute for Microbial Diseases (RIMD), Osaka University;Department of Genome Informatics, RIMD, Osaka University;National Institute of Health, Department of Medical Sciences

关键词: dengue virus;    sequence diversity;    primary infection;    secondary infection;    quasispecies;   

DOI  :  10.7883/yoken.67.132

学科分类：传染病学

来源: National Institute of Infectious Diseases

【 摘 要 】

References(15)Dengue fever (DF) and dengue hemorrhagic fever (DHF) are caused by mosquito-borne dengue virus (DENV) infection leading to death in tropical and subtropical countries. In Thailand, all 4 serotypes of DENV are circulating. The most severe cases of DF and DHF are primarily introduced by secondary infections. Epidemiological studies have demonstrated that approximately 20% of the primary infection cases were caused by DENV-1 and -3, while the cases of DENV-2 or -4 accounted for less than 3%. For this reason, DENV-2 and -4 from primary infections have not been well studied. In this study, the sequence diversity of the envelope gene of 8 DENV-2 clinical isolates from primary/secondary infections was analyzed. DENV-2 from primary infections were highly heterogeneous in individual patients, whereas those from secondary infections were homogeneous. Phylogenetic analysis demonstrated that the heterogeneous population of DENV-2 from primary infections was composed of closely related quasispecies. Homogenous DENV-2 could be derived from selection of a particular viral population in secondary infections. The degree of sequence diversity of DENV-2 varied, and thus quasispecies may be involved in the progression of DENV infection.

【 授权许可】

Unknown