Partial Cloning of Prohibitin cDNA from Canine, Feline, Bovine, Equine, and Rabbit Liver mRNA by RT-PCR

Journal of Veterinary Medical Science

Kihei KUBO¹  Yasuhiko TAKAMORI¹  Fumihito OHASHI²  Satoshi MATSUYAMA¹ 

[1] Laboratory of Radiology, Department of veterinary Science, College of Agriculture, Osaka Prefecture University;Laboratory of Veterinary Surgery, Department of Veterinary Science, College of Agriculture, Osaka Prefecture University

关键词: prohibitin;    RT-PCR;    tumor suppressor gene;   

DOI  :  10.1292/jvms.59.201

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(11)Cited-By(7)Prohibitin is the protein which has an inhibitory function in cell growth, and its gene is suggested to be one of putative tumor suppressor genes. In this report, we described a partial cloning of prohibitin cDNAs from canine, feline, bovine, equine, and rabbit liver mRNAs by RT-PCR, and their homology analysis. The sequences of these RT-PCR products were compared with each other as well as those reported for human and rat. The homology in this region of prohibitin cDNA was approximately 90%, and the amino acid sequence of each RT-PCR product shared more than 95% identity. Therefore, it is concluded that all the RT-PCR products are a part of prohibitin cDNA of each animal.

【 授权许可】

Unknown