Serial Changes of CD4+CD25+FoxP3+ Regulatory T Cell in Canine Model of SepsisInduced by Endotoxin

Journal of Veterinary Medical Science

Jinho PARK¹  Dohyeon YU²  Junhwan KIM¹  Chul PARK¹ 

[1] College of Veterinary Medicine, Chonbuk National University, Jeonju 561�?756, Korea;College of Veterinary Medicine, Chonnam National University, Gwangju 500�?757, Korea

关键词: canine endotoxemia model;    immunosuppression;    lipopolysaccharide (LPS);    regulatory T cell;    sepsis;   

DOI  :  10.1292/jvms.13-0586

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(23)Regulatory T cells (Tregs) suppress the immune system and maintain thehomeostasis of the immune system in healthy dogs. In septic patients, the percentage ofcirculating Tregs is increasing, which may cause the sepsis-induced immunosuppression.This study was performed to investigate the changes of the percentage of Tregs in totallymphocytes of the peripheral blood in the experimental canine endotoxemia model. Theanimals injected with a high dose lipopolysaccharide (LPS) induced severe leukopeniafollowed by leukocytosis, but the total lymphocytes number was relatively consistent. As aresult of flow cytometric analysis, the percentage of Tregs in total lymphocytes of theperipheral blood was 8.45 ± 1.30% (day 0), and it temporarily decreased 2.54 ± 1.16% (day1) and increased continuously until the end of the experiment (14.34 ± 4.10% on day 3 and25.70 ± 7.39% on day 7), respectively. This study provides basic information inphysiologic and immunologic changes in Tregs in dogs with sepsis model.

【 授权许可】

Unknown