Detection of Salmonella by Surface Plasmon Resonance

Sensors

Benjamin Barlen¹  Saikat Datta Mazumdar¹  Olga Lezrich¹  Peter Kämpfer² 

[1] Institut für Pharmazeutische Chemie, Philipps-Universität Marburg, Marbacher Weg 6, D-35032 Marburg, Germany; E-mails:;Institut für Angewandte Mikrobiologie, Justus-Liebig-Universität Gieβen, Heinrich-Buff-Ring 26-32, D-35392 Gieβen, Germany; E-mail:

关键词: Salmonella serovars;    lipopolysaccharide (LPS);    O-specific antibody;    sequential detection;    Plasmonic^® surface plasmon resonance (SPR) device;   

DOI  :  10.3390/s7081427

来源: mdpi

【 摘 要 】

This study explores the possibility of simultaneous and specific detection of Salmonella serovars by surface plasmon resonance (SPR). The Plasmonic^® SPR device was used to develop this rapid assay. The sandwich immunoassay involves the use of a polyclonal anti-Salmonella antibody to simultaneous capture multiple Salmonella serovars present in a sample. This is followed by specific detection of the captured serovars using O-specific anti-Salmonella antibodies. Milk spiked with Salmonella Typhimurium and Salmonella Enteritidis was used as a model system to establish the assay. The assay was further extended to sequentially differentiate between the two Salmonella serovars on a single SPR chip in a single channel. The assay was proved to work without any additional dilution or clean-up steps. The sample volume requirement for the assay is only 10 μL. The lower limits of detection for Salmonella Typhimurium and Salmonella Enteritidis were 2.50×10⁵ cells mL⁻¹ and 2.50×10⁸ cells mL⁻¹, respectively.

【 授权许可】

CC BY   
© 2007 by the authors; licensee MDPI, Basel, Switzerland.