Localized Expression of Histamine H1 Receptors in Syncytiotrophoblast Cells of Human Placenta

Journal of Pharmacological Sciences

Kazuto Matsuyama¹  Hiroyuki Fukui¹  Yuichi Nitta¹  Shuhei Horio¹  Yoshio Ikoma³  Tatsuya Ichikawa¹  Kazutaka Ishimura² 

[1] Department of Molecular Pharmacology, Institute of Health and Biosciences, the University of Tokushima, Japan;Department of Anatomy and Cell Biology, Institute of Health and Biosciences, the University of Tokushima, Japan;Department of Industry-Academia Collaboration Research Planning, Intellectual Property Office, the University of Tokushima, Japan

关键词: histamine H1 receptor;    mepyramine;    placenta;    syncytiotrophoblast;    immunohistochemistry;   

DOI  :  10.1254/jphs.FP0060862

学科分类：药学

来源: Nihon Yakuri Gakkai Henshuubu / Japanese Pharmacological Society

【 摘 要 】

References(43)Cited-By(2)The previous Northern blot analysis and in situ hybridization studies showed that histamine H1-receptor (H1R) mRNA is expressed in human placenta and suggested that H1R plays some roles in the function of placenta in pregnancy. To investigate further, it is essential to show the precise location of H1R in the placenta. In the present study, we investigated H1R expression in human placenta by radioligand binding assay and immunohistochemical study using an antibody against human H1R. Placentas were obtained from normal uncomplicated deliveries. Membranes prepared from the tissue exhibited saturable [3H]mepyramine binding (Kd = 4.0 ± 0.6 nM and Bmax = 91.4 ± 4.9 fmol/mg of protein). Stereoisomers of chlorpheniramine inhibited [3H]mepyramine binding; d-chlorpheniramine inhibited more potently than l-chlorpheniramine, Ki values being 1.1 ± 0.4 and 270 ± 170 nM, respectively. The placenta tissues were positively immunostained with anti-H1R antibody only in the region of the syncytiotrophoblast of chorionic villus. The tissues were double stained with anti-H1R antibody and an antibody against human chorionic gonadotoropin (hCG) that is solely expressed in placental syncytiotrophoblast cells. The results showed that H1R and hCG were expressed on the same cells, that is, syncytiotrophoblast cells. These results indicate that H1Rs are specifically expressed in syncytiotrophoblast cells of human placenta organ.

【 授权许可】

Unknown