Identification and Carbohydrate Specificity of a Chicken Serum Mannan-Binding Protein Reactive with a Ra Chemotype Strain of Salmonella Typhimurium

Journal of Veterinary Medical Science

Shunji SUGII¹  Yoshikazu HIROTA¹ 

关键词: chicken;    MBP;    RaRF;    Salmonella Typhimurium;    serum protein;   

DOI  :  10.1292/jvms.56.747

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(25)Cited-By(5)To compare a chicken serum mannan-binding protein (MBP) with a chicken Ra-reactive factor (RaRF), both serum proteins were isolated by combination of different chromatography. In SDS-PAGE, both purified proteins were resolved into a major protein band with a molecular weight of 34, 000 and a few minor protein bands. In Western blotting with both purified proteins, only a single band with a molecular weight of 34, 000 was detected with anti-chicken serum MBP antibody, indicating that the major protein band of chicken serum MBP and RaRF are antigenically identical. The combining sites of these two proteins were most reactive with N-acetylmannosamine followed by mannose, N-acetylglucosamine, and L-fucose. These findings suggest that chicken serum MBP may be identical to its corresponding bactericidal factor RaRF in terms of antigenicity and monosaccharide specificity.

【 授权许可】

Unknown