Detection of Bacillus cereus isolated during ultra high temperature milk production flowchart through random amplified polymorphic DNA polymerase chain reaction

Ciência Rural

Cardoso, Marita Vedovelli¹  Abreu, Irlan Leite de²  Gonçalves, Ana Carolina Siqueira¹  D'Abreu, Léa Furlan³  Rossi, Gabriel Augusto Marques¹  Rossi Junior, Oswaldo Durival¹  Bürger, Karina Paes¹  Vidal, Ana Maria Centola⁴ 

[1] Universidade Estadual Paulista, Jaboticabal, Brazil;Centro Universitário de Rio Preto, São José do Rio Preto, Brasil;USP, Pirassununga, Brazil;Universidade de São Paulo, Pirassununga, Brazil

关键词: Bacillus cereus;    enterotoxins;    microbiological quality;    pasteurized milk;    raw milk;    UHT milk;   

DOI  :  10.1590/0103-8478cr20141539

学科分类：农业科学（综合）

来源: Universidade Federal de Santa Maria * Centro de Ciencias Rurais

【 摘 要 】

:The present study focused on isolation Bacillus cereus during the UHT milk production and shelf life, to assess the enterotoxigenic production capacity of isolates and to evaluate the use of the RAPD-PCR technique to verify whether Bacillus cereus isolated at different phases of UHT milk processing belongs to the same strain. For this, six groups of milk samples composed of raw, pasteurized and UHT milk were collected from a processing plant. The results revealed that bacteria belonging to the Bacillus cereus group were isolated from 51.6%, 81.6% and from 13.8% of raw, pasteurized and UHT milk samples, respectively. About 50.0% of isolates from raw milk, 19.2% isolates from pasteurized milk and 70.7% isolates from UHT milk were capable of producing enterotoxins. It was confirmed the genetic similarity among Bacillus cereus isolates from raw, pasteurized and UHT milk, therefore demonstrating that the microorganism is able to withstand UHT treatment. These results should serve as a warning to health authorities, given that 13.8% of samples were not in accordance with standards established by the Department of Health for containing a potentially pathogen agent, therefore indicating that contamination of milk by sporulating bacteria should be avoided.

【 授权许可】

Unknown