Evaluation of the live vaccine efficacy of virulence plasmid-cured, and phoP- or aroA-deficient Salmonella enterica serovar Typhimurium in mice

Journal of Veterinary Medical Science

Hidenori MATSUI¹  Yohsuke OGAWA³  Yasunori ISSHIKI²  Masahiro EGUCHI³  Yoshihiro SHIMOJI³ 

[1] Kitasato Institute for Life Sciences and Graduate School of Infection Control Sciences, Kitasato University, 5�?9�?1 Shirokane, Minato-ku, Tokyo 108-8641, Japan;Department of Microbiology, School of Pharmaceutical Sciences, Josai University, Sakado, Saitama 350-0295, Japan;National Institute of Animal Health, National Agriculture and Food Research Organization, 3�?1�?5 Kannondai, Tsukuba, Ibaraki 305-0856, Japan

关键词: aroA;    BALB/c mouse;    oral vaccine;    phoP;    Salmonella enterica serovar Typhimurium;   

DOI  :  10.1292/jvms.14-0013

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(34)Cited-By(4)We evaluated the protective efficacy of 94-kb virulence plasmid-cured, and phoP- or aroA-deficient strains of Salmonella enterica serovar Typhimurium (ΔphoP or ΔaroA S. Typhimurium) as oral vaccine candidates in BALB/c mice. Two weeks after the completion of 3 oral immunizations with 1 × 108 colony-forming units (CFU) of virulence plasmid-cured, and ΔphoP or ΔaroA S. Typhimurium at 10-day intervals, S. Typhimurium lipopolysaccharide (LPS)-specific mucosal secretory immunoglobulin A (s-IgA) antibody titers were detected in the cecal homogenate, bile and lung lavage fluid, but not in the intestinal lavage fluid. In addition, the increases in S. Typhimurium LPS-specific immunoglobulin G (IgG) and IgA antibody titers in the serum were also observed 2 weeks after completing 3 oral immunizations with virulence plasmid-cured, and ΔphoP or ΔaroA S. Typhimurium. The series of 3 oral immunizations protected the mice against an oral challenge with 5 × 108 CFU of the virulent strain of S. Typhimurium, suggesting that both the virulence plasmid-cured, and ΔphoP and ΔaroA S. Typhimurium strains are promising candidates for safe and effective live S. Typhimurium vaccines.

【 授权许可】

Unknown