Cryopreservation of the Semen Collected by Electroejaculation from the Hokkaido Brown Bear (Ursus arctos yesoensis)

Journal of Veterinary Medical Science

Motozumi MATSUI¹  Yoshiyuki TAKAHASHI¹  Akiko ISHIKAWA¹  Hideyuki SAKAMOTO²  Seiji KATAGIRI¹ 

[1] Laboratory of Theriogenology, Graduate School of Veterinary Medicine, Hokkaido University;Noboribetsu Bear Park

关键词: brown bear;    freezing;    spermatozoa;   

DOI  :  10.1292/jvms.64.373

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(17)Cited-By(22)Four adult Hokkaido brown bears were used as semen donors, and semen characteristics were examined before freezing and after thawing. A total of 10 electroejaculates were diluted with Tris-egg yolk extender and cooled to 4°C over 90 min. Spermatozoa were equilibrated with 4.7% glycerol for 80 min. Semen packed in 0.25 ml plastic straws were frozen with liquid nitrogen vapor. Percentages (mean ± SD) of motile and live sperm were 96 ± 2 and 86.5 ± 7.2% before freezing, and 43 ± 5 and 67.4 ± 3.9% after thawing, respectively. Although the number of progressively motile sperm after thawing varied among samples (1.8 ± 1.2 × 108 cells/ejaculate), frozen semen in the present study might serve for artificial insemination.

【 授权许可】

Unknown