Granulocyte-Colony Stimulating Factor Augments Neovascularization Induced by Bone Marrow Transplantation in Rat Hindlimb Ischemia

Journal of Pharmacological Sciences

Hiroshi Iwao²  Takashi Omura¹  Kazuhide Takeuchi¹  Yasuhiro Takagi¹  Ryo Matsumoto¹  Soichiro Enomoto¹  Minoru Yoshiyama¹  Kaname Akioka¹  Daisuke Nishiya¹  Junichi Yoshikawa¹  Takanori Kusuyama¹ 

[1]Department of Internal Medicine and Cardiology, Osaka City University Graduate School of Medical Sciences

[2]Department of Pharmacology, Osaka City University Graduate School of Medical Sciences

关键词: granulocyte-colony stimulating factor;    bone marrow-mononuclear cell transplantation;    angiogenesis;    hindlimb ischemia;    endothelial progenitor cell;   

DOI  :  10.1254/jphs.FP0040966

学科分类：药学

来源: Nihon Yakuri Gakkai Henshuubu / Japanese Pharmacological Society

【 摘 要 】

References(35)Cited-By(12)Because granulocyte-colony stimulating factor (G-CSF) mobilizes bone marrow cells including endothelial progenitor cells, we examined whether G-CSF augments angiogenesis and collateral vessel formation induced by bone marrow-mononuclear cells transplantation (BMT). Unilateral hindlimb ischemia was surgically induced in Lewis rats. One week after surgery, administration of 100 mg/kg per day G-CSF significantly increased the laser Doppler blood perfusion index (LDBPI), number of angiographically detectable collateral vessels (angiographic score), and capillary density determined by alkaline phosphatase staining. In the BMT group (1 × 107 cells/rat) and the group with combined G-CSF treatment and BMT, LDBPI was significantly increased compared with that in the vehicle-treated group. In the BMT group, neovascularization was significantly increased as evidenced by the angiographic score and capillary density compared with the vehicle-treated group. Furthermore, the combination of G-CSF treatment and BMT augmented neovascularization compared with BMT alone, as evidenced by the angiographic score and capillary density. Moreover, G-CSF significantly increased vascular endothelial growth factor mRNA and fibroblast growth factor-2 mRNA in hindlimb muscle. In conclusion, G-CSF was found to augment neovascularization in rat hindlimb ischemia. Combined use of G-CSF treatment and BMT may be a useful strategy for therapeutic neovascularization in ischemic tissues.

【 授权许可】

Unknown