Variation from Cytopathogenic Biotype to Non-Cytopathogenic Biotype is Correlated with the Deletion of Cellular Sequence from Bovine Viral Diarrhea Viruses

Journal of Veterinary Medical Science

Yoshihiro SAKODA¹  Nobuo OGAWA²  Shigeyuki NAKAMURA²  Kenichi SAKAMOTO¹  Akio FUKUSHO¹  Tomoaki SHIMAZAKI²  Yoshimitsu INOUE² 

[1] National Institute of Animal Health, Department of Exotic Diseases;National Veterinary Assay Laboratory

关键词: BVDV;    cytopathogenicity;    pestivirus;    RT-PCR;   

DOI  :  10.1292/jvms.59.361

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(28)Cited-By(5)Non-cytopathogenic (NCP) viruses of bovine viral diarrhea (BVD) virus were detected at a low ratio by the reverse plaque formation method from virus samples after several plaque clonings of cytopathogenic (CP) BVD viruses; NADL and Osloss strains. This phenomenon suggests that the NCP BVD viruses are produced at a low ratio during the propagation of CP BVD viruses in vitro. To investigate the differences between the parent CP BVD virus and the NCP BVD virus as a real progeny, the regions flanking the insertion of cellular mRNA in the p125 domain of NADL and Osloss strains were amplified by RT-PCR and cloned into pGEM 3Z plasmid vector, and then sequenced. Consequently, it was confirmed that sequences of cellular mRNA insertion of CP BVD viruses, NADL and Osloss strains, were completely and exactly deleted from the NCP BVD viruses which were real progeny of CP BVD viruses, NADL and Osloss strains. These results suggest that NCP BVD viruses may revert from CP biotype to NCP biotype by the deletion of cellular mRNA insertion in the viral genome of CP BVD viruses (NADL and Osloss strains).

【 授权许可】

Unknown