Grapevine yellows diseases in Spain: eight year survey of disease spread and molecular characterization of phytoplasmas involved

Anales del Jardín Botánico de Madrid

Simona Botti²  Josep Rahola³  María P. Martín⁴  Assunta Bertaccini²  Ester Torres¹ 

[1] Laboratori de Sanitat Vegetal, DARP, Generalitat de Catalunya, Barcelona;DiSTA, Patologia Vegetale, University of Bologna, Bologna;Servei Sanitat Vegetal, DARP, Generalitat de Catalunya;Real Jardín Botánico, Madrid

关键词: Flavescence dorée;    bois noir;    molecular detection;    Vitis;   

DOI  :  

学科分类：植物学

来源: Jardin Botanico de Madrid. Anales

【 摘 要 】

Among grapevine yellows phytoplasma diseases in Europe, flavescence dorée (FD) is the most devastating and in the last decade has reached Spanish vineyards, mainly in Catalonia. An eight-year survey was carried out in the areas where the disease has spread (Alt Empordà, Catalonia, Northern Spain) and in the remaining vine-growing areas of Catalonia. Sequence analyses of a portion of the 16S-23S ribosomal DNA cistron, from selected grapevine samples from Catalonia, showed that the phytoplasmas involved in grapevine yellows belong to 16S ribosomal subgroups V-D (flavescence dorée, FD) and XII-A (bois noir, BN). A set of Spanish FD isolates collected during these years were further studied by RFLP analyses of the 16S-23S ribosomal DNA fragment, as well as the rpS3 and SecY genes. All the FD phytoplasma strains studied were related to phytoplasmas belonging to ribosomal protein subgroup rp-E.

【 授权许可】

Unknown