Effect of Active Immunization of Pony Mares against Recombinant Porcine Inhibin α Subunit on Ovarian Follicular Development and Plasma Steroids and Gonadotropins

Journal of Veterinary Medical Science

Yoh-Ichi MIYAKE¹  M Shafiqul HOQUE¹  Yoshihisa MAEDA¹  Gen WATANABE²  Takeshi OSAWA¹  Refaat Ibrahim DERAR¹  Kazuyoshi TAYA² 

[1] Laboratory of Theriogenology, Department of Veterinary Medicine, Faculty of Agriculture, Iwate University;Laboratory of Veterinary Physiology, Department of Veterinary Medicine, Faculty of Agriculture, Tokyo University of Agricultural Technology

关键词: follicular development;    FSH;    immunization;    inhibin;    mare;   

DOI  :  10.1292/jvms.66.31

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(23)Cited-By(4)Two pony mares were immunized against recombinant porcine inhibin α subunit three times with 39 day intervals. Clinical findings and endocrinological changes before immunization were taken as the control. The first significant rise in the anti-inhibin titre (P<0.05) in the circulation was found 27 days after the first injection. Maximum binding activity was reached by the 12th day after the second booster dose. The number of small, medium and large sized follicles had increased significantly compared to before immunization (11.75 ± 4.30, 2.75 ± 0.69 and 2.51 ± 0.63 vs 6.50 ± 1.43, 1.83 ± 0.44 and 1.33 ± 0.38, respectively), but the ovulation rate remained unchanged after immunization. The average plasma concentration of FSH and estradiol-17β during the estrous cycle increased significantly (P<0.05) after immunization. These results suggest that immunization against inhibin is a useful tool to increase the number of ovarian follicles during the estrous cycle of pony mares. Moreover, the present study supported the concept that inhibin plays a major role in the control of follicular growth through its inhibitory effect on FSH secretion synergistically with steroid hormones.

【 授权许可】

Unknown