Characterization of vpr Vector Constructed from Chimeric Simian and Human Immunodeficiency Virus

Journal of Veterinary Medical Science

Masashi OKOBA²  Takeshi HAGA²  Yuya SHIMIZU²  Shino KUMABE²  Masanori HAYAMI¹  Takeo KUWATA¹  Tomoyuki MIURA¹  Yoshitaka GOTO²  Nanase YAMAZAKI²  Iouri L. KOZYREV¹ 

[1] Laboratory of Viral Pathogenesis, Institute for Virus Research, Kyoto University;Department of Veterinary Microbiology, Miyazaki University

关键词: SHIV;    vector;    vpr;   

DOI  :  10.1292/jvms.65.633

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(13)Cited-By(1)Chimeric simian and human immunodeficiency viruses (SHIVs) are useful tool for investigating AIDS pathogenesis and for development of vaccine. We constructed a SHIV-vpr vector (designated as SHIV-3sj) by replacing vpr region with restriction enzyme sites. SHIV-3sj was designed to express inserted gene along with its viral replication. Five cytokine genes were inserted into SHIV-3sj, and ability of viral replication and expression of the inserted genes were examined. The short insert including RANTES and IL-5 resulted in the successful expression from SHIV-3sj, while the construct having longer genes including IL-2, IL-6 and IL-12p35 failed to become replication competent. These results suggest that the length of the insert is an important factor for the replication ability of SHIV-3sj vector.

【 授权许可】

Unknown