Inducers and autoinducers on Salmonella enterica serovar Typhimurium motility, growth and gene expression

Ciência Rural

Sturbelle, Régis Tuchtenhagen¹  Conceição, Rita de Cássia dos Santos da¹  Leite, Fábio Pereira Leivas¹  Timm, Cláudio Dias¹ 

[1] Universidade Federal de Pelotas, Pelotas, Brazil

关键词: Quorum sensing;    Salmonella;    epinephrine;    autoinducer;    motilityRESUMOSalmonella está entre os principais micro-organismos patogênicos veiculados por alimentos;   

DOI  :  10.1590/0103-8478cr20150295

学科分类：农业科学（综合）

来源: Universidade Federal de Santa Maria * Centro de Ciencias Rurais

【 摘 要 】

Genus Salmonella bacteria are among the major pathogenic microorganisms in food. This bacterium pathogenicity is related to a number of virulence factors, among which its flagella. Flagellum expression is one of the virulence factors modulated by Quorum Sensing. Epinephrine produced by mammals uses the same signaling pathway of the 3 bacteria autoinducer. This study evaluated the effect of molecules inducer (epinephrine) and autoinducers (autoinducer 2 and autoinducer 3) and their association with the motility, growth and expression genes flhC, fliA, fliY, motA, motB e fliC of Salmonella Typhimurium (ST). Initially, ST was inoculated in BHI. Then, motility assays, growth curves and gene expression were performed by testing different concentrations of epinephrine (50, 125, 250, 500µM), conditioned medium (10 and 50%) and a combination of these. ST was exposed to different concentrations of epinephrine, conditioned medium and an association of both. Following, motility assays, bacterial growth and gene expression were performed. The results obtained showed that the combination of 500uM epinephrine with 50% conditioned medium increased ST bacterial motility by increasing the expression of genes involved in flagellum assembly.

【 授权许可】

Unknown