Assessment of antioxidant potential of Mollinedia racemosa (romadizo)

Revista Cubana de Plantas Medicinales

Solanilla Duque, José Fernando¹  Lombo, Oscar¹  Méndez Arteaga, Jonh Jairo¹  Murillo Perea, Elizabeth¹ 

[1] Departamento de Producción y Sanidad Vegetal, Ibagué, Colombia

关键词: Mollinedia racemosa;    antioxidant activity;    DPPH;    ABTS;    lipid peroxidation;    phenolic compounds;   

DOI  :  

学科分类：药理学

来源: Editorial Ciencias Medicas

【 摘 要 】

Introduction: Mollinedia racemosa (Schltdl.) Tul. (romadizo), is one species used by indigenous and peasant communities in the Colombian Orinoco basin, to treat fever, headache and stomach problems, especially in the treatment of cold and as an analgesic. Objective: to evaluate the antioxidant potential of M. racemosa (romadizo) leaves. Methods: ethanolic macerate of leaves was prepared (ratio 1:15, plant/solvent), changing the solvent every 24 h until the sample was exhausted. The extract was filtered, concentrated under reduced pressure and stored (4°C). The content of phenolic compounds, flavonoids and total flavonols were assessed. The concentration of alkaloids of this plant was also established. Additionally, the in vitro antioxidant activity of plant was determined by measuring the ability of ethanol to stabilize radicals DPPH1 (1-diphenil-2-picrilhydracil) and ABTS (2,2'azinobis-[3 etilbenzotiazolin]-6-sulphonic acid) and the potential to inhibit lipid peroxidation. Results: the antioxidant potential of this plant depended on the applied concentration that in turn was correlated to the diversity and quantity of phenols present in the plant. Concentration rates of 14.59 and 17.32 µg/mL were required to inhibit 50 % (IC50) of the original quantity of DPHH and ABTS respectively. The inhibitory capacity of lipidic peroxidation reached its maximum level after 360 hours (15 days). Conclusions: the results showed that the extract from M. racemosa leaves had antioxidant and protective effect in a lipidic system.

【 授权许可】

CC BY-NC