Establishment and Characterization of Canine Rhabdomyosarcoma Cell Line CMS-C

Journal of Veterinary Medical Science

Masaki MICHISHITA³  Daigo AZAKAMI⁴  Marie DOHI⁴  Mariko TAKASAKI⁴  Hana SHIBUTANI⁴  Hitoshi HATAKEYAMA²  Akihiro MORI⁴  Makoto BONKOBARA⁵  Toshinori SAKO⁴  Katsumi ISHIOKA⁴  Seigo OGASAWARA¹  Yutaka MOMOTA⁴  Tsukimi WASHIZU⁵ 

[1] Department of Population Medicine and Diagnostic Sciences, College of Veterinary Medicine, Cornell University;Laboratory of Comparative Cellular Biology, Faculty of Veterinary Science, Nippon Veterinary and Life Science University;Department of Veterinary Pathology, Faculty of Veterinary Science, Nippon Veterinary and Life Science University;Department of Veterinary Nursing, Faculty of Veterinary Science, Nippon Veterinary and Life Science University;Department of Veterinary Clinical Pathology, Faculty of Veterinary Science, Nippon Veterinary and Life Science University

关键词: canine;    novel cell lines;    rhabdomyosarcoma;   

DOI  :  10.1292/jvms.10-0436

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(16)Cited-By(1)A novel canine tumor cell line designated as the CMS-C cell line was established from pleomorphic rhabdomyosarcoma (RMS) raised in the prostate gland of a 14-year-old intact male mixed-breed dog. CMS-C cells displayed the same immunohistochemical characteristics (positive for vimentin and desmin and negative for cytokeratin and smooth muscle actin) as the original tumor cells and express myoD1 and UCP3, known as striated muscle-specific molecules, as shown by RT-PCR assay. Therefore, the established CMS-C cell line appears to be of rhabdomyoblast cell origin. The CMS-C cell line established from pleomorphic RMS will be a useful tool for further studies about canine RMS.

【 授权许可】

Unknown