Molecular Typing by Random Amplification of Polymorphic DNA (RAPD) and Detection of Virulence Genes of Salmonella enterica subspecies enterica serovar Gallinarum biovar Gallinarum

Journal of Veterinary Medical Science

Dong-Seok LEE²  Tae-Wook HAHN²  Sun-Joong KIM¹  Ji-Dong JIN²  Eun-Kyung SHIN²  Rose JUNG³ 

[1] College of Veterinary Medicine, Seoul National University;School of Veterinary Medicine, Kangwon National University;School of Pharmacy, University of Colorado HSC

关键词: RAPD;    Salmonella Gallinarum;    universal rice primers;    virulence gene;   

DOI  :  10.1292/jvms.68.1321

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(26)Cited-By(6)Salmonella enterica subspecies enterica serovar Gallinarum biovar Gallinarum is the causative agent of fowl typhoid in chickens, outbreaks of which have devastated poultry populations in Korea since 1992. In order to identify genetic differences among S. Gallinarum isolates, bacteria were examined using the random amplified polymorphic DNA (RAPD) method. Of 13 arbitrary primers screened initially, the primer designated as universal rice primer-6 (URP-6) was selected for subsequent typing assays because it produced a distinctive and reproducible DNA fingerprint for a S. Gallinarum reference strain. URP-6-based RAPD analysis assigned 30 S. Gallinarum isolates into 6 types, with 26 isolates (86.6%) belonging to 2 major RAPD types. The distribution of virulence genes in S. Gallinarum isolates was examined by Southern hybridization. All tested isolates had the invasion gene, invA, the virulence plasmid gene, spvB, and the S. Enteritidis fimbrial gene, sefC. The distribution of virulence genes among S. Gallinarum isolates did not correlate with any specific RAPD type.

【 授权许可】

Unknown