Molecular differentiation between Salmonella enterica subsp enterica serovar Pullorum and Salmonella enterica subsp enterica serovar Gallinarum

Brazilian Journal of Microbiology

Simone Alves Mendes Ribeiro²  Jaqueline Boldrin De Paiva¹  Fábio Zotesso¹  Manoel Victor Franco Lemos¹  Ângelo Berchieri Júnior¹ 

[1] ,Laboratório Nacional Agropecuário Ministério da Agricultura, Pecuária e Abastecimento Campinas SP ,Brasil

关键词: Salmonella Pullorum;    Salmonella Gallinarum;    differentiation;    PCR;    Salmonella Pullorum;    Salmonella Gallinarum;    diferenciação;    PCR;   

DOI  :  10.1590/S1517-83822009000100032

来源: SciELO

【 摘 要 】

S. Pullorum (SP) and S. Gallinarum (SG) are very similar. They are the agents of pullorum disease and fowl typhoid, respectively, and the two diseases are responsible for economic losses in poultry production. Although SP and SG are difficult to be differentiated in routine laboratory procedures, the ability to metabolize ornithine is a biochemical test that may be used to achieve this aim. While SP is able to decarboxylate this amino acid, SG is not. However, the isolation of strains showing atypical biochemical behavior has made this differentiation difficult. One of the genes associated with the metabolization of the amino acid ornithine is called speC, and is found in both serovars. The analysis of 21 SP and 15 SG strains by means of PCR did not enable the differentiation of the two serovars, because fragments produced were identical. However, after enzymatic treatment with restriction enzyme Eco RI, the band pattern of each serovar showed to be different, even in samples of atypical biochemical behavior. This fact enabled the standardization of the technique for a quick and safe differentiation of serovars Pullorum and Gallinarum.

【 授权许可】

CC BY-NC   
 All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License