Role of β1 Integrins in Adhesion of Canine Mastocytoma Cells to Extracellular Matrix Proteins

Journal of Veterinary Medical Science

Tomoko TAKAHASHI³  Takayuki NAKAGAWA¹  Nobuo SASAKI¹  Ryohei NISHIMURA¹  Manabu MOCHIZUKI²  Emi OHASHI¹ 

[1] Laboratory of Veterinary Surgery, Graduate School of Agricultural and Life Sciences, The University of Tokyo;Laboratory of Veterinary Diagnostic Imaging, Department of Veterinary Medicine, Faculty of Agriculture, Tokyo University of Agriculture and Technology;Laboratory of Comprehensive Veterinary Clinical Studies, Department of Veterinary Medicine, College of Bioresource Sciences, Nihon University

关键词: canine;    extracellular matrix;    integrin β1;    subfamily;    mastocytoma cell;   

DOI  :  10.1292/jvms.69.495

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(24)Cited-By(1)The interactions of tumor cells with the extracellular matrix (ECM) are a crucial step in invasion and metastasis. Integrins are adhesive molecules forming heterodimers with α and β subunits that play a definitive role in these interactions. In this study, mastocytoma (mast cell tumor: MCT) cell-ECM interaction was investigated using 3 canine MCT cell lines: CM-MC (originating from cutaneous MCT), VI-MC (originating from intestinal MCT), and CoMS (originating from oral MCT). Flow cytometric analysis showed that all cells highly expressed the integrin β1 and α1 through α5 subunits and that they moderately expressed the α6 subunit. In adhesion studies, CoMS weakly but spontaneously adhered to fibronectin (FN), which was enhanced by phorbol ester (TPA), while CM-MC and VI-MC required cell activation by TPA to adhere to FN. Anti-β1 and α5 integrin antibodies strongly inhibited cell adhesion to FN in CM-MC and CoMS and moderately inhibited cell adhesion in VI-MC. Only VI-MC adhered to laminin (LN) under activation by TPA. Anti-β1 integrin antibodies strongly inhibited cell adhesion to LN, but all anti-α integrin antibodies failed to inhibit cell adhesion to LN. No cells adhered to collagen types I and IV. Canine MCT cells from different origins expressed similar integrin patterns; however, there were some differences in adhesive behavior in response to various ECM proteins and activating stimuli.

【 授权许可】

Unknown