Molecular Cloning of Canine Membrane-Anchored Inhibitor of Matrix Metalloproteinase, RECK

Journal of Veterinary Medical Science

Takanori KITAMURA¹  Masahiro OKUMURA³  Toru FUJINAGA³  Yoshinao HOSAKA²  Satoshi TAKAGI³  Tsuyoshi KADOSAWA³  Tomohiro OHSAKI³  Darko BOSNAKOVSKI³ 

[1] Department of Pharmacology, Graduate School of Medicine, Kyoto University;Department of Veterinary Anatomy, School of Veterinary Medicine, Rakuno Gakuen University;Laboratory of Veterinary Surgery, Department of Clinical Science, Graduate School of Veterinary Medicine, Hokkaido University

关键词: canine;    extracellular matrix;    proteinase inhibitor;    RECK;    tumor malignancy;   

DOI  :  10.1292/jvms.67.385

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(22)Cited-By(6)The reversion-inducing cysteine-rich protein with Kazal motifs (RECK) gene is one of the endogenous matrix metalloproteinase (MMP) inhibitors. It was reported that decreased RECK expression closely correlated with tumor malignancy. We determined the cDNA sequence of the canine RECK gene. The cDNA sequence and deduced amino acid of canine RECK were 2,913 bases and 971 residues, respectively. The predicted amino acid sequence of the protein showed 95.5% and 91.9% homology with human and mouse RECK, respectively. RECK mRNA expression was analyzed in various canine tissues and tumor cell lines by quantitative RT-PCR. The highest RECK expression was detected in lung and testis. In comparison with the tissues, a remarkably low expression level was detected in tumor cell lines. In addition, the RECK gene was transfected in the canine transitional cell carcinoma, and its influence on cell proliferation, migration, and invasion was analyzed. The transfected RECK gene suppressed only canine tumor invasion. These results showed that RECK might play an important role in tumor malignancy in dogs as well as in other mammalians.

【 授权许可】

Unknown