Overproduction of the Pro-Apoptotic Molecule, Programmed Cell Death 5, in Toxoplasma gondii Leads to Increased Apoptosis of Host Macrophages

Journal of Veterinary Medical Science

Jun-ichi WATANABE³  Kyoko YAMADA¹  Osamu KAWASE¹  Hany Mohamed IBRAHIM¹  Yoshifumi NISHIKAWA¹  Hiroshi BANNAI¹  Xuenan XUAN¹  Chihiro SUGIMOTO² 

[1] National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine;Research Center for Zoonosis Control, Hokkaido University;The Institute of Medical Science, The University of Tokyo

关键词: apoptosis;    overexpression;    PDCD5;    Toxoplasma gondii;   

DOI  :  10.1292/jvms.71.1183

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(39)Cited-By(2)We established a recombinant strain of Toxoplasma gondii that overexpressed programmed cell death 5 (TgPDCD5), in order to evaluate the role of endogenous TgPDCD5 in macrophage apoptosis during T. gondii infection. Immunofluorescence microscopy revealed that overproduced TgPDCD5 with a hemagglutinin tag was localized in the cytosol, which was consistent with the localization of endogenous TgPDCD5. The induced TgPDCD5-HA was recognized as an additional band by Western blot analysis, indicating successful overexpression of TgPDCD5. Secretion and release of TgPDCD5 by the parasite was also up-regulated in a time-dependent manner, which reflected its overproduction. Apoptosis due to parasite infection and interferon-gamma treatment was significantly up-regulated by the overexpression of TgPDCD5. These results suggest that endogenous TgPDCD5 plays a role in macrophage apoptosis during T. gondii infection.

【 授权许可】

Unknown