Detection of nodavirus in seawater from rearing facilities for Atlantic halibut Hippoglossus hippoglossus larvae

Diseases of Aquatic Organisms

Audun H. Nerland¹  Cecilie Skaar¹  Tove Boge Eriksen¹  Hogne Bleie¹ 

关键词: Nodavirus;    RT-PCR;    Real-time PCR;    Quantification;    Seawater;    Atlantic halibut;   

DOI  :  10.3354/dao073201

学科分类：生物科学（综合）

来源: Inter-Research

【 摘 要 】

ABSTRACT: We used (1) ultracentrifugation followed by RT-PCR and (2) real-time RT-PCR to detect and quantify nodaviruses in seawater in which Atlantic halibut Hippoglossus hippoglossus larvae/fry had been held at rearing facilities. Evaluated against in vitro propagated viruses, the viral concentration corresponded to 1.6 × 10⁴ TCID₅₀ (50% tissue culture infectious dose) ml^–1. Evaluated against in vitro transcribed RNA, the concentration was estimated at 2 × 10⁷ virus particles ml^–1 seawater.

【 授权许可】

Unknown