Occurrence of bovine ephemeral fever in Okinawa Prefecture, Japan, in 2012and development of a reverse-transcription polymerase chain reaction assay to detectbovine ephemeral fever virus gene

Journal of Veterinary Medical Science

Tomoko KATO²  Kazufumi IKEMIYAGI³  Tohru YANASE²  Tsuyoshi NIWA³  Hiroaki SHIRAFUJI²  Moemi SUZUKI³  Yoshiki NITTA¹ 

[1] Yaeyama Livestock Hygiene Service Center, Okinawa Prefectural Government, 1�?2 Miyara, Ishigaki 907-0022, Japan;Kyushu Research Station, National Institute of Animal Health, National Agriculture and Food Research Organization, 2702 Chuzan, Kagoshima 891-0105, Japan;Okinawa Prefectural Institute of Animal Health, 24�?29 1-chome, Kohagura, Naha, Okinawa 900-0024, Japan

关键词: arbovirus;    bovine ephemeral fever;    diagnosis;    molecular epidemiology;    RT-PCR;   

DOI  :  10.1292/jvms.14-0492

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(20)Cited-By(4)In September 2012, several cows and a calf showed decreased activity, anorexia and feveron Ishigaki Island, Okinawa Prefecture, Japan, and the cases were diagnosed as bovineephemeral fever (BEF). We isolated BEF virus (BEFV) from one of the affected cows and thendetermined the complete genome sequence of the G gene, which encodes a class Itransmembrane glycoprotein of BEFV. The BEFV isolate in this case, ON-3/E/12, was sortedinto the same cluster as other BEFV isolates in Japan, Taiwan and China obtained in1996�?2004 and was most closely related to a 2002 Chinese isolate, JT02L, according to thephylogenetic analysis of the complete G gene. Since inactivated vaccines for BEF availablein Japan are considered effective against the ON-3/E/12 isolate as well as other isolatesin East Asia from 1996�?2004, annual vaccination should be conducted to prevent BEF inOkinawa. Additionally, in this study, we developed an RT-PCR assay to detect the BEFV genein Japan and neighboring countries. Our assay was able to amplify target sequences in allof the tested BEFV isolates, including 18 isolates in Japan and another isolate inAustralia. The assay was found to be useful also for testing RNA samples extracted frombovine peripheral blood mononuclear cells, and the detection limit of the assay was 10copies per tube. We believe that our assay would be an important tool for the screening ofBEFV infection and the diagnosis of BEF.

【 授权许可】

Unknown