期刊论文详细信息
Diseases of Aquatic Organisms
Development of monoclonal antibodies fordetection of necrotizing hepatopancreatitisin penaeid shrimp
Deborah J. Bradley-Dunlop1  Donald V. Lightner1  Carlos Pantoja1 
关键词: NHP;    Necrotizing hepatopanceatitis;    Monoclonal antibodies;    Dot-immunoblot;    Immunohistochemistry;   
DOI  :  10.3354/dao060233
学科分类:生物科学(综合)
来源: Inter-Research
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【 摘 要 】

ABSTRACT: Monoclonal antibodies (MAbs) were produced against necrotizing hepatopancreatitis bacteria (NHP-B) of penaeid shrimp. The MAbs tested in dot-immunoblot (D-IB) assays were capable of detecting the NHP-B in hepatopancreas samples collected frommoribund juvenile Litopenaeus vannamei during an experimentally induced NHP-B infection. The MAbs were also screened by immunohistochemistry (IHC) using case submissions that were determined to be infected not only by histology, but also polymerasechain reaction (PCR) and in situ hybridization (ISH) assays using specific digoxigenin (DIG)-labeled probes on histological sections prepared from naturally infected shrimp. Two of the MAbs were chosen for development of detection methods for NHP.The MAbs were tested using IHC methods on Davidson’s alcohol-formalin-acetic acid (AFA) fixed tissue sections and identified NHP-B infected cells and tissues in a pattern similar to that seen with DIG-labeled NHP-specific gene probes. None of theMAbs reacted with tissue from specific pathogen-free (SPF) shrimp or with shrimp tissues infected with a rickettsia-like bacteria, Vibrio sp., Campylobacter sp., and Spiroplasma sp. The MAbs were found to be negativeagainst these other organisms, demonstrating that they are species specific and useful for rapid diagnostic detection of NHP-B.

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