Improvement of a PCR method for the detection of necrotizing hepatopancreatitis in shrimp

Diseases of Aquatic Organisms

Donald V. Lightner¹  Carlos Pantoja¹  Linda M. Nunan¹ 

关键词: PCR;    Necrotizing hepatopancreatitis;    NHP;    Shrimp;    Penaeus vannamei;   

DOI  :  10.3354/dao01908

学科分类：生物科学（综合）

来源: Inter-Research

【 摘 要 】

ABSTRACT: Necrotizing hepatopancreatitis (NHP) is considered to be one of the most important bacterial diseases affecting penaeid shrimp culture and is caused by an unclassified Gram-negative, pleomorphic, intracellular Alphaproteobacterium. Due to the enteric nature of the bacteria, PCR is the one non-lethal method available for detection of the pathogen. Over a decade ago, a PCR protocol was developed for detection of NHP, which over the subsequent years was shown to occasionally generate false positive reactions. The University of Arizona Aquaculture Pathology Laboratory has developed a set of primers and PCR cycling parameters that have been tested on a variety of DNA templates, using 2 types of PCR reagent systems, which eliminated the generation of false positive amplicons.

【 授权许可】

Unknown