RANDOMLY AMPLIFIED POLYMORPHIC DNA-A BRIEF REVIEW | Science Publications

American Journal of Animal and Veterinary Sciences

Nandani Kumari¹  Saroj Kumar Thakur¹ 

关键词: DNA;    PCR;    Polymorphism;    Dendrogram;    Genetic Diversity;   

DOI  :  10.3844/ajavsp.2014.6.13

学科分类：兽医学

来源: Science Publications

【 摘 要 】

RAPD is a PCR based technique which involves the use of single arbitrary short primers (8-12 nucleotides), resulting in the amplification of many discrete DNA. The segments of DNA that are amplified are random. The technique was developed independently by two different laboratories and called as RAPD and AP-PCR (Arbitrary Primed PCR). This procedure detects nucleotide sequence polymorphisms in a DNA amplification based assay using only a single primer of arbitrary nucleotide sequence. The RAPD technology has provided a quick and efficient screen for DNA-sequence polymorphisms at a very large no of loci. The present communication gives emphasis on basic knowledge about RAPD, procedure, its advantages disadvantages, limitations and applications of RAPD.

【 授权许可】

Unknown