Flow Cytometric Analysis of Canine Umbilical Cord Blood Lymphocytes

Journal of Veterinary Medical Science

Akira ISHIKAWA¹  Kiichi KANAYAMA²  Takeki YAMADA¹  Shigehisa TSUMAGARI³  Kohei OHTA³  Tadatoshi OHTAKI³  Isao OTANI²  Tsuyoshi ISHINAZAKA³ 

[1] Kameido Animal Hospital;Laboratory of Veterinary Physiology, Department of Veterinary Medicine, College of Bioresource Sciences, Nihon University;Laboratory of Theriogenology, Department of Veterinary Medicine, College of Bioresource Sciences, Nihon University

关键词: canine;    lymphocyte;    umbilical cord;   

DOI  :  10.1292/jvms.70.285

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(17)Cited-By(4)Lymphocyte subsets in canine umbilical cord blood were flow cytometrically analyzed and compared with those of the dams' peripheral blood. The proportion of CD3+ T lymphocytes, CD21+CD3- B lymphocytes, and CD3-CD21- non-T non-B lymphocytes in umbilical cord blood was 52.9%, 30.4%, and 16.7%, respectively. T lymphocyte/B lymphocyte ratio was significantly lower in the umbilical cord blood than in the dams' peripheral blood (2.1 ± 1.4 versus 11.0 ± 8.1, P < 0.001). In contrast, CD4+ lymphocyte/CD8+ lymphocyte ratio was significantly higher in the umbilical cord blood than in the dams' peripheral blood (7.6 ± 2.2 versus 1.8 ± 0.6, P<0.001). These findings clarified the phenotypic characters of canine umbilical cord blood lymphocytes.

【 授权许可】

Unknown