Purification and Quantification of Lactoferrin in Equine Seminal Plasma

Journal of Veterinary Medical Science

Masami INAGAKI¹  Yohji OHNAMI²  Koichi ORINO¹  Motohiro KIKUCHI²  Kiyotaka WATANABE¹ 

[1] Laboratory of Biochemistry, School of Veterinary Medicine and Animal Sciences, Kitasato University;Laboratory of Theriogenology, School of Veterinary Medicine and Animal Sciences, Kitasato University

关键词: equine;    lactoferrin;    seminal plasma;   

DOI  :  10.1292/jvms.64.75

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(20)Cited-By(10)Lactoferrin with a molecular mass of 80 kDa was purified from equine seminal plasma by heparin-Agarose affinity chromatography and Sephacryl S-200 gel filtration. Purified lactoferrin was found to be highly homogeneous on the bases of its migration as a single band on sodium dodecyl sulfate-polyacrylamide gel electrophoresis and of the monospecificity of rabbit antibodies to the purified protein in immunoblotting of seminal plasma proteins. A sandwich enzyme-linked immunosorbent assay was developed for quantifying lactoferrin in equine seminal plasma. Seminal plasma lactoferrin concentrations in 23 normal stallions ranged from 42 to 453 μg/ml, with a mean value of 157 ± 118 μg/ml (S.D.).

【 授权许可】

Unknown