Experimental bacteriophage-mediated virulence in strains of Vibrio harveyi

Diseases of Aquatic Organisms

James Munro¹  Jane Oakey¹  Erin Bromage¹  Leigh Owens¹ 

关键词: Vibriosis;    Vibrio harveyi;    Bacteriophage;    Haemolysin;    Protein synthesis;    Monoclonal antibodies;    Penaeus monodon;   

DOI  :  10.3354/dao054187

学科分类：生物科学（综合）

来源: Inter-Research

【 摘 要 】

ABSTRACT: Vibriosis is a major disease problem in prawn aquaculture. Until now there has been no clear explanation why some strains of Vibrio are pathogenic, while others are not. This study demonstrated that the presence of the bacteriophage V.harveyi myovirus like (VHML) may confer virulence to V. harveyi Strain 642. This was demonstrated by infecting naïve avirulent V. harveyi Strains 12, 20, 45 and 645 with the bacteriophage and converting them into virulent strains. Thepreviously naïve strains of Vibrio infected with Bacteriophage VHML from V. harveyi Strain 642 demonstrated up-regulation of haemolysin, up-regulation of protein excretion, additional proteins which were recognised as toxic proteins fromStrain 642 by monoclonal antibodies specific to the exotoxin sub-units, and a significant increase in mortality of larval Penaeus monodon. It was concluded that Bacteriophage VHML conferred virulence to V. harveyi Strains 12, 20, 45 and 645and that Bacteriophage VHML either fully or partly confers virulence in V. harveyi Strain 642.

【 授权许可】

Unknown