Endocrine Journal | |
Detection of Thyroid-Stimulating Antibody Using Frozen Stocks of Chinese Hamster Ovary Cells Transfected with Cloned Human Thyrotropin Receptor | |
HIROMI SUMIZAKI1  NOBUYUKI AMINO1  TORU TAKANO1  | |
[1] Department of Laboratory Medicine, Osaka University Medical School | |
关键词: Thyroid-stimulating antibody; CHO; Graves' disease; Cyclic AMP; | |
DOI : 10.1507/endocrj.44.431 | |
学科分类:内分泌与代谢学 | |
来源: Japan Endocrine Society | |
【 摘 要 】
References(13)Cited-By(5)To measure thyroid-stimulating antibody (TSAb) in sera from patients with Graves' disease, we developed a new assay system with using frozen stocks of CHO-K1 cells. CHO-K1 cells trasfected with cloned thyrotropin (TSH) receptor on a 96 well plate were frozen in Cell BankerTM and stored at -70°C. Three days before the assay, they were thawed in the culture medium and allowed to grow in a monolayer until use. The medium was replaced with medium containing IgGs from the patients, then after 2h, it was collected and concentrations of adenosine 3'-5'-cyclicmonophosphate (cAMP) were measured. This method is sensitive enough to detect TSAb and it is simpler and easier than the methods which use FRTL-5 cells.
【 授权许可】
Unknown
【 预 览 】
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