Development of a Loop-Mediated Isothermal Amplification Method for DetectingStreptococcus equi subsp. zooepidemicus and Analysisof Its Use with Three Simple Methods of Extracting DNA from Equine Respiratory TractSpecimens

Journal of Veterinary Medical Science

Yoshinari KATAYAMA¹  Yuta KINOSHITA¹  Hidekazu NIWA¹ 

[1] Microbiology Division, Epizootic Research Center, Equine Research Institute, Japan Racing Association, 1400�?4 Shiba, Shimotuke, Tochigi 329�?0412, Japan

关键词: DNA extraction method;    equine;    LAMP method;    Loopamp PURE DNA Extraction Kit;    Streptococcus equi subsp. zooepidemicus;   

DOI  :  10.1292/jvms.14-0140

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(26)Cited-By(6)Streptococcus equi subsp. zooepidemicus(S. zooepidemicus) is a dominant pathogenic bacterium in equinepneumonia. We developed a specific loop-mediated isothermal amplification (LAMP) method,which targets the gene encoding sorbitol-6-phosphate 2-dehydrogenase(sorD), for detecting S. zooepidemicus and examinedthe clinical efficacies of its use in combination with each of 3 DNA extraction methodseasily used by veterinary practitioners, namely the Loopamp PURE DNA Extraction Kit,InstaGene Matrix and a conventional boiling method. The LAMP method plus the Loopamp PUREDNA Extraction Kit gave higher rates of positivity than the other combinations in bothclinical and spiked samples containing clinically significant concentrations (>1 ×104 CFU/ml) of S. zooepidemicus.

【 授权许可】

Unknown