Antimicrobial peptides discovered in theblack tiger shrimp Penaeus monodon usingthe EST approach

Diseases of Aquatic Organisms

Ikuo Hirono¹  Rath Pichyangkura¹  Sirawut Klinbunga¹  Anchalee Tassanakajon¹  Takashi Aoki¹  Premruethai Supungul¹ 

关键词: Antimicrobial peptides;    Penaeus monodon;    Hemocytes;    RT-PCR;    EST;   

DOI  :  10.3354/dao061123

学科分类：生物科学（综合）

来源: Inter-Research

【 摘 要 】

ABSTRACT: Two cDNA libraries were prepared from hemocytes of normal and Vibrio harveyi-challenged black tiger shrimp Penaeus monodon. A total of 1062 expressed sequence tag (EST) clones were sequenced unidirectionally. ESTs representing theantimicrobial peptide (AMP) homologues, antilipopolysaccharide factors (ALF), penaeidins and crustins were discovered. They predominated among immune-related genes, representing 29.2% and 64.0% of the normal and challenged libraries, respectively. Severaltypes of each AMP homologue were found. Sequence alignments of ALF in P. monodon (ALFPm 1 to 5) implied possible alternative splicing of different exons at both NH₂ and COOH-termini. Only one major type of penaeidin(penPm3) was found in P. monodon. In addition, crustin homologues (crusPms1 to 4) and a newly identified glycine-rich antibacterial peptide (GAMPPm1) were also isolated and characterized. Using RT-PCR analysis, expression ofALF, penaeidin and crustin transcripts was detected in various tissues but the main expression site was in hemocytes. Expression of these antimicrobial peptides in P. monodon subjected to V. harveyi challenge revealed a significant increasein expression of ALFPms (p < 0.05) but a decrease in expression of crustins and penaeidins.

【 授权许可】

Unknown