Serofendic Acid Promotes Stellation Induced by cAMP and cGMP Analogs in Cultured Cortical Astrocytes

Journal of Pharmacological Sciences

Akinori Akaike³  Hachiro Sugimoto¹  Tetsuhiro Niidome¹  Toshiaki Kume³  Ryo Ito³  Ryota Taguchi³  Yasuhiko Izumi³  Hiroshi Katsuki⁴  Yuki Takada-Takatori² 

[1] Department of Neuroscience for Drug Discovery Research, Graduate School of Pharmaceutical Sciences, Kyoto University, Japan;Department of Pharmacology, Faculty of Pharmaceutical Sciences, Doshisha Women’s College, Japan;Department of Pharmacology, Graduate School of Pharmaceutical Sciences, Kyoto University, Japan;Department of Chemico-Pharmacological Sciences, Graduate School of Pharmaceutical Sciences, Kumamoto University, Japan

关键词: serofendic acid;    astrocyte;    stellation;    cAMP;    cGMP;   

DOI  :  10.1254/jphs.08254FP

学科分类：药学

来源: Nihon Yakuri Gakkai Henshuubu / Japanese Pharmacological Society

【 摘 要 】

References(41)Cited-By(4)We investigated the effect of serofendic acid, a neuroprotective substance derived from fetal calf serum, on the morphological changes in cultured cortical astrocytes. Cultured astrocytes developed a stellate morphology with several processes following exposure to dibutylyl cAMP (dbcAMP), a membrane-permeable cAMP analog; 8-Br-cGMP, a membrane-permeable cGMP analog; or phorbol-12-myristate-13-acetate (PMA), a protein kinase C activator. Serofendic acid significantly accelerated the stellation induced by dbcAMP- and 8-Br-cGMP. In contrast, the PMA-induced stellation was not affected by serofendic acid. Next, we attempted to elucidate the mechanism underlying the dbcAMP-induced stellation and explore the site of action of serofendic acid. Both the stellation induced by dbcAMP and the promotional effect of serofendic acid were partially inhibited by KT5720, a specific protein kinase A (PKA) inhibitor. Furthermore, serofendic acid failed to facilitate the stellation induced by Y-27632, an inhibitor of Rho-associated kinase (ROCK). These results indicate that serofendic acid promotes dbcAMP- and 8-Br-cGMP-induced stellation and the promotional effect on dbcAMP-induced stellation is mediated at least partly by the regulation of PKA activity and not by controlling ROCK activity.

【 授权许可】

Unknown