Proliferative Responses of a Bovine Leukemia Virus-Infected Lymphoblastoid B-Cell Line by Its Culture Supernatant and Cytokines

Journal of Veterinary Medical Science

Atsuhiko HASEGAWA¹  Hajime TSUJIMOTO¹  Ryo GOITSUKA¹  YANG Mhan-Pyo¹ 

关键词: BLV-infected lymphoblastoid B-cell line;    cytokine;    proliferation;   

DOI  :  10.1292/jvms.54.255

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(17)Cited-By(2)The growth-promoting activity in the culture supernatant of bovine lymphoblastoid B-cell lines (BL2M3 and BL312) were examined. BL2M3 cells proliferated well in response to conditioned medium (CM) obtained from BL2M3 and BL312 cell cultures. These BL2M3 and BL312 CM were used as sources of BL2M3 cell growth-promoting factor (BL2M3-GPF). BL2M3-GPF was sensitive to acid (pH 2) and alkali (pH 10) and was heat-labile. Proliferative responses of BL2M3 cel1s were not induced by human recombinant (r)IL 1, rIL 2, rIL 6, granulocyte-colony stimulating factor (rG-CSF) or tumor necrosis factor (rTNF)-α. Human low molecular weight B cel1-growth factor (LMW-BCGF) was, however, capable of augmenting the proliferation of BL2M3 cel1s. BL2M3 cells formed clusters in response to LMW-BCGF, whereas they showed single and discete appearance in the presence of BL2M3-GPF. These results suggested that bovine lymphoblastoid B-cell lines might release and respond to the growth-promoting factor for in vitro proliferation of its own cell line, BL2M3.

【 授权许可】

Unknown