Relation between hemolysis with presence and quantification of immunoglobulin in red blood cells in cases of autoimmune hemolytic anemia

Revista Cubana de Hematología, Inmunología y Hemoterapia

Ávila-Cabrera, Onel M.¹  Bencomo-Hernández, Antonio A.²  Jaime-Fagundo, Juan C.¹  Espinosa-Martínez, Edgardo¹  Hernández-Ramírez, Porfirio¹  Alfonso-Valdés, María E.¹ 

[1] Instituto de Hematología e Inmunología, Ciudad de La Habana, Cuba

关键词: : Autoimmune hemolytic anemia;    autoantibodies;    IgG subclasses;    quantification;    ELISA;   

DOI  :  

学科分类：生理学与病理学

来源: SciELO

【 摘 要 】

A group of 135 patients was studied: 111 adults and 24 children presenting hot autoimmune hemolytic anemia (HAIHA) The erythrocyte characterization and the number of immunoglobulin molecules by red blood cells was determined by ELISA. In 51 patients the pattern of IgG subclasses was investigated. The hemolysis intensity was classified by the hemoglobin concentration, the reticulocytes count and the figures of plasma haptoglobin. In patients presenting with high degree hemolysis IgM autoantibodies were detected in combination with IgG, IgA isotypes. In cases only with the presence of IgG, the number of molecules of autoantibodies by red blood cells was the determinant factor in hemolysis. There were not differences in relation to subclasses pattern. The severity of hemolysis in HAIHA is related to the coexistence of multiple immunoglobulins in red blood cells and especially of the IgM.

【 授权许可】

CC BY-NC