電荷�?28残基周期性を持ったタンパク質の核�?在予測

Chem-Bio Informatics Journal

�? 閏聡¹  美宅 成樹¹  崎山 則征¹  澤田 隆介¹  園山 正史¹ 

[1] 名古屋大学大学院工学研究科

关键词: proteome;    プロテオーム;    charge distribution;    電荷分布;    nuclear localization protein;    核局在タンパク質;    prediction;    予測;    bioinformatics;    バイオインフォマティクス;   

DOI  :  10.1273/cbij.7.35

学科分类：生物化学/生物物理

来源: Chem-Bio Informatics Society

【 摘 要 】

References(12)Cited-By(2)Proteins with a charge periodicity of 28 residues (PCP28) were found recently in the human proteome, and many of the annotated PCP28 were located in the nucleus (Ke et al., Jpn. J. Appl. Phys. 2007). The physical properties of the amino acid sequences were analyzed to detect the difference in the physicochemistry between the nuclear and cytoplasmic PCP28 and develop a software system to classify the two types of PCP28. A significant difference in the global parameters from the entire sequence and the local parameters around a segment with the highest positive charge density was found between the nuclear and cytoplasmic PCP28. The global classification score included the densities of proline and cysteine, and the negative charge density, while the local score included the symmetry of the charge distribution, the density of cysteine, and the positive charge density. A prediction system was developed using the global and local scores, which possessed a sensitivity and specificity of 92% and 88%, respectively. The mechanism of translocation of proteins to the nucleus is discussed using the parameters relevant to the predictive system.

【 授权许可】

Unknown